Anti-EAAT2 Antibody (A12711)

Name: Anti-EAAT2 Antibody
Brand: Antibodies.com
SKU: A12711
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to EAAT2 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Astrocyte Marker

Specifications

Name

Anti-EAAT2 Antibody

Description

Rabbit polyclonal antibody to EAAT2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human EAAT2/EAAT2/SLC1A2 (NP_004162.2).

Sequence

HLSKSELDTIDSQHRVHEDIEMTKTQSIYDDMKNHRESNSNQCVYAAHNSVIVDECKVTLAANGKSADCSVEEEPWKREK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Excitatory amino acid transporter 2, GLT1, Glutamate/aspartate transporter II, SLC1A2, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-EAAT2 Antibody (A12711)

Western blot analysis of Mouse brain, using Anti-EAAT2 Antibody (A12711) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-EAAT2 Antibody (A12711)

Immunofluorescence analysis of mouse brain cells using Anti-EAAT2 Antibody (A12711) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

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