Anti-E2F1 (phospho Thr433) Antibody (A94933)

Name: Anti-E2F1 (phospho Thr433) Antibody
Brand: Antibodies.com
SKU: A94933
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to E2F1 (phospho Thr433) for WB and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-E2F1 (phospho Thr433) Antibody

Description

Rabbit polyclonal antibody to E2F1 (phospho Thr433).

Specificity

This antibody detects endogenous levels of E2F1 only when phosphorylated at Thr433.

Applications

WB, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:5000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide derived from human E2F1 around the phosphorylation site of Thr433 (amino acids 388-437).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

46kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E2F-1, PBR3, pRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (1)

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Western Blot - Anti-E2F1 (phospho Thr433) Antibody (A94933)

Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h using Anti-E2F1 (phospho Thr433) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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