Rabbit polyclonal antibody to E2F1 (Phospho-Thr433)
Specificity
The antibody detects endogenous level of E2F1 only when phosphorylated at Threonine 433.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Threonine 433 (D-L-T(p)-P-L) derived from Human E2F1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
E2F-1, PBR3, pRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Western blot analysis of extracts from Mouse brain tissue using E2F1 (Phospho-Thr433) Antibody #12006.The lane on the left is treated with the antigen-specific peptide.
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