Anti-E2F1 Antibody [ARC0058] (A308217)

Name: Anti-E2F1 Antibody [ARC0058]
Brand: Antibodies.com
SKU: A308217
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0058] antibody to E2F1 for WB and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-E2F1 Antibody [ARC0058]

Description

Rabbit monoclonal [ARC0058] antibody to E2F1.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F1 (Q01094).

Sequence

PPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0058

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E2F-1, PBR3, pRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-E2F1 Antibody [ARC0058] (A308217)

Western blot analysis of extracts of various cell lines, using Anti-E2F1 Antibody [ARC0058] (A308217) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-E2F1 Antibody [ARC0058] (A308217)

Western blot analysis of extracts of rat spleen, using Anti-E2F1 Antibody [ARC0058] (A308217) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Western Blot - Anti-E2F1 Antibody [ARC0058] (A308217)

Immunoprecipitation analysis of 300µg extracts of HT-29 cells using 3µg of Anti-E2F1 Antibody [ARC0058] (A308217). This Western blot was performed on the immunoprecipitate using Anti-E2F1 Antibody [ARC0058] (A308217) at a dilution of 1:1000.

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