Anti-E2F1 Antibody (A11509)

Name: Anti-E2F1 Antibody
Brand: Antibodies.com
SKU: A11509
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to E2F1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-E2F1 Antibody

Description

Rabbit polyclonal antibody to E2F1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:20-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-301 of human E2F1 (NP_005216.1).

Sequence

GGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E2F-1, PBR3, pRB-binding protein E2F-1, RBAP-1, RBBP-3, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-E2F1 Antibody (A11509)

Western blot analysis of extracts of various cell lines, using Anti-E2F1 Antibody (A11509) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Immunofluorescence - Anti-E2F1 Antibody (A11509)

Immunofluorescence analysis of C6 cells using Anti-E2F1 Antibody (A11509) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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