Anti-E Cadherin Antibody [SPM471] - BSA and Azide free (A254007)

Anti-E Cadherin Antibody [SPM471] - BSA and Azide free

Mouse monoclonal [SPM471] antibody to E Cadherin.

This antibody recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as -catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Flow Cytometry, IF, WB, IHC-P

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml

Human

This antibody does not cross react with Mouse or Rat.

Recombinant human E Cadherin protein.

Mouse

Monoclonal

SPM471

IgG1

Protein A/G chromatography.

1 mg/ml

120-80 kDa (mature) / 135 kDa (Precursor)

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-E Cadherin Antibody [SPM471] (A250827).

Cadherin-1, CAM 120/80, CD324, CDH1, CDHE, Epithelial cadherin, UVO, Uvomorulin