Recombinant human monoclonal antibody to E Cadherin.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse
Host
Human
Clonality
Monoclonal
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 146.6 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-E Cadherin Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A324002) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Anti-E Cadherin Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A324002) confirms that the purity of this antibody is greater than 95%.
Immobilized recombinant human CDH1 protein (His tag) at 2 µg/ml is bound by Anti-E Cadherin Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A324002). EC50 = 0.004381 µg/ml.
Publishing research using Recombinant Anti-E Cadherin Antibody [Research Grade Biosimilar] - Low endotoxin, Azide free (A324002)? Please let us know so that we can list the citation on this page.