Anti-E Cadherin Antibody (A80540)

Name: Anti-E Cadherin Antibody
Brand: Antibodies.com
SKU: A80540
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to E Cadherin for WB, IHC-P, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-E Cadherin Antibody

Description

Rabbit polyclonal antibody to E Cadherin.

Applications

WB, IHC-P, ICC/IF, ELISA

Positive Control

HCT116, Mouse liver.

Dilutions

WB: 1:500-1:1,000, IHC-P: 1:50-1:100, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 373-622 of human E Cadherin.

Sequence

PIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPN

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

125 kDa / 135 kDa

Predicted MW

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cadherin-1, CAM 120/80, CD324, CDH1, CDHE, Epithelial cadherin, UVO, Uvomorulin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-E Cadherin Antibody (A80540)

E Cadherin expression in HCT116 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-E Cadherin Antibody (A80540) at a dilution of 1:400 and detected with chemiluminescence.

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Western Blot - Anti-E Cadherin Antibody (A80540)

E Cadherin expression in Mouse liver lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-E Cadherin Antibody (A80540) at a dilution of 1:1500 and detected with chemiluminescence.

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Immunohistochemistry - Anti-E Cadherin Antibody (A80540)

E Cadherin expression in rat kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM Tris/EDTA buffer, pH 9.0. Staining was performed with Anti-E Cadherin Antibody (A80540) at a dilution of 1:50.

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Immunofluorescence - Anti-E Cadherin Antibody (A80540)

E Cadherin expression in A431 cells analyzed by immunofluorescence. Staining was performed with Anti-E Cadherin Antibody (A80540) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).