This antibody recognises CD324 also known as E-Cadherin, a transmembrane glycoprotein which mediates calcium dependant cell adhesion and cell junction formation. The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions. The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells. This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).
Applications
Flow Cytometry, IHC-Fr, IP, WB, ICC
Dilutions
Flow Cytometry: 1:10 - 1:20, IHC-Fr: 1:125 - 1:250, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Human
Immunogen
T-47D cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
67A4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Purity
>95% (by SDS-PAGE).
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze!
General Notes
Mouse anti Human CD324, clone 67A4 recognizes CD324 also known as E-Cadherin, a transmembrane glycoprotein which mediates calcium dependant cell adhesion and cell junction formation. The extracellular domain mediates a Ca2+ dependant homophillic interaction with a cadherin molecule on the surface of a neighboring cell. This leads to the formation of tight junctions which assist in blocking the movement of cells and facilitating cell-cell interactions (Gloushankova et al. 2017). The cytoplasmic domain is able to interact with catenins which link the CD324 to the actin cytoskeleton which help mediate downstream signaling events growth inhibitory signals to prevent uncontrolled tissue growth during embryonic development, tissue regeneration, wound healing. Loss of CD324 expression has been associated with tumor progression and metastasis. It is thought that metastasis is more likely to occur due to the loss of a maintained stable physical link between tumor cells (Mendonsa et al. 2018). This clone 67A4 has been used in immunocytochemistry experiments to examine the localization of CD324 on medullary epithelial cells (Kutleša et al. 2002).