Rabbit polyclonal antibody to Dopamine Transporter.
Specificity
This antibody recognises an epitope within the C-terminal region of dopamine transporter (DAT), also known as solute carrier family 6.DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine.The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
Applications
WB, IHC-Fr
Dilutions
IHC-Fr: 1:1,000, WB: 1:1,000
Reactivity
Human, Monkey, Mouse
Immunogen
Synthetic peptide corresponding to the C-terminus of human dopamine transporter, conjugated to Keyhole Limpet Haemocyanin.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Molecular Weight
Approximately 88 kDa in human caudate cell lysates.
Product Form
Liquid
Formulation
Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human dopamine transporter antibody recognizes an epitope within the C-terminal region of dopamine transporter (DAT), also known as solute carrier family 6. DAT is principally expressed in the presynaptic membrane of dopaminergic nerve terminals, where it mediates the rapid removal of dopamine from the synaptic cleft after neuronal stimulation. As such, DAT plays an important role in regulating the availability of dopamine in the synaptic cleft and thus in modulating the physiological functions of dopamine. The reuptake of dopamine by DAT is inhibited by psychostimulants such as cocaine and amphetamine.
Synonyms
DA transporter, DAT, DAT1, SLC6A3, Sodium-dependent dopamine transporter, Solute carrier family 6 member 3
