Anti-DNMT1 Antibody [60B1220.1] (A304715)

Western blot analysis of human 293T cell lysates showing detection of ~180kDa DNMT1 protein using Anti-DNMT1 Antibody [60B1220.1] (A304715) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Marker. Lane 2: human 293T Cell lysate. Load: 15 µg. Block: 1.5% BSA in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~180kDa. Other Band(s): Lower bands are DNMT1 degradation.

Immunohistochemistry analysis of mouse medullar kidney tissue. The Primary Antibody used was Anti-DNMT1 Antibody [60B1220.1] (A304715) at 1:1,000. The secondary antibody used was HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.

Western blot analysis of human H1299 cell lysate showing detection of DNMT1 protein using Anti-DNMT1 Antibody [60B1220.1] (A304715) at 1:1,000.

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-DNMT1 Antibody [60B1220.1] (A304715) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Magnification: 40x. ABC solution 1:1,000 dilution, DAB solution 100-200uL/ slide.