Anti-DNA PKcs Antibody (A11446)

Name: Anti-DNA PKcs Antibody
Brand: Antibodies.com
SKU: A11446
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to DNA PKcs for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-DNA PKcs Antibody

Description

Rabbit polyclonal antibody to DNA PKcs.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 3993-4128 of human DNA-PKcs (NP_008835.5).

Sequence

SDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

450 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNPK1, HYRC, HYRC1, p460, PRKDC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-DNA PKcs Antibody (A11446)

Western blot analysis of extracts of various cell lines, using Anti-DNA PKcs Antibody (A11446) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-DNA PKcs Antibody (A11446)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-DNA PKcs Antibody (A11446) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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