Anti-DMT1 Antibody (A12850)

Name: Anti-DMT1 Antibody
Brand: Antibodies.com
SKU: A12850
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to DMT1 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-DMT1 Antibody

Description

Rabbit polyclonal antibody to DMT1.

Applications

WB, IHC

Dilutions

WB: 1:1,000-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human DMT1/SLC11A2 (NP_001167597.1).

Sequence

MVLGPEQKMSDDSVSGDHGESASLGNINPAYSNPSLSQSPGDSEEYFATYFNEKISIPEEEYS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DCT1, Divalent cation transporter 1, Divalent metal transporter 1, DMT-1, Natural resistance-associated macrophage protein 2, NRAMP 2, NRAMP2, SLC11A2, Solute carrier family 11 member 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-DMT1 Antibody (A12850)

Western blot analysis of extracts of various cell lines, using Anti-DMT1 Antibody (A12850) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-DMT1 Antibody (A12850)

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-DMT1 Antibody (A12850) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-DMT1 Antibody (A12850)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-DMT1 Antibody (A12850) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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