Anti-DLL4 Antibody (A283083)

Anti-DLL4 Antibody

Rabbit polyclonal antibody to DLL4.

This antibody recognises human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72 kDa containing a single DSL domain and eight EGF-like domains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor, Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 is strongly expressed in tumor vessels of primary renal tumors , bladder cancer , and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity. Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemisrtry on FFPE tissues using rabbit anti human DLL4 antibody.

WB, IHC-P, ELISA

ELISA: 1:3,000 - 1:15,000, IHC-P: 5 - 15 µg/ml, WB: 1:300 - 1:1500

Human, Mouse, Rat, Chimpanzee

Synthetic peptide corresponding to the internal region of human DLL4.

Rabbit

Polyclonal

IgG

Approximately 74 kDa in pancreatic cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Human Delta-like Protein 4 antibody detects human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72 kDa containing a single DSL domain and eight EGF-like domains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor, Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network (Mailhos et al. 2001). DLL4 is strongly expressed in tumor vessels of primary renal tumors (Patel et al. 2005), bladder cancer (Patel et al. 2006), and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity (Ridgway et al. 2006).Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemisrtry on FFPE tissues using rabbit anti human DLL4 antibody (Gunin et al. 2014).

Delta-like protein 4, Delta4, Drosophila Delta homolog 4