Anti-Desmoplakin Antibody (A12154)

Name: Anti-Desmoplakin Antibody
Brand: Antibodies.com
SKU: A12154
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Desmoplakin for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Desmoplakin Antibody

Description

Rabbit polyclonal antibody to Desmoplakin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Desmoplakin (NP_004406.2).

Sequence

MSCNGGSHPRINTLGRMIRAESGPDLRYEVTSGGGGTSRMYYSRRGVITDQNSDGYCQTGTMSRHQNQNTIQELLQNCSDCLMRAELIVQPELKYGDGIQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

330 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Desmoplakin Antibody (A12154)

Western blot analysis of extracts of various cell lines, using Anti-Desmoplakin Antibody (A12154) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-Desmoplakin Antibody (A12154)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Desmoplakin Antibody (A12154) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Desmoplakin Antibody (A12154)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Desmoplakin Antibody (A12154) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Desmoplakin Antibody (A12154)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Desmoplakin Antibody (A12154) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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