Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Damage-specific DNA-binding protein 1, DDB p127 subunit, DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, HBV X-associated protein 1, UV-damaged DNA-binding factor, XAP-1, XAP1
Western Blot - Anti-DDB1 Antibody [ARC1278] (A308971)
Western blot analysis of extracts of various cell lines, using Anti-DDB1 Antibody [ARC1278] (A308971) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-DDB1 Antibody [ARC1278] (A308971) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-DDB1 Antibody [ARC1278] (A308971) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-DDB1 Antibody [ARC1278] (A308971) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western Blot - Anti-DDB1 Antibody [ARC1278] (A308971)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-DDB1 Antibody [ARC1278] (A308971). This Western blot was performed on the immunoprecipitate using Anti-DDB1 Antibody [ARC1278] (A308971) at a dilution of 1:3000.