Anti-DDB1 Antibody (A83757)

Name: Anti-DDB1 Antibody
Brand: Antibodies.com
SKU: A83757
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to DDB1 for ELISA, WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Specifications

Name

Anti-DDB1 Antibody

Description

Goat polyclonal antibody to DDB1.

Applications

ELISA, WB, IHC

Dilutions

ELISA: 1:32,000, WB: 1-2µg/ml, IHC: 4-6µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the C terminal region of human DDB1.

Sequence

C-DLIKVVEELTRIH

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

140 kDa

Predicted MW

126.9kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Damage-specific DNA-binding protein 1, DDB p127 subunit, DDBa, DNA damage-binding protein 1, DNA damage-binding protein a, HBV X-associated protein 1, UV-damaged DNA-binding factor, XAP-1, XAP1

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-DDB1 Antibody (A83757)

DDB1 expression in HeLa (A), HepG2 (B) and Jurkat (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DDB1 Antibody (A83757) at 1µg/ml and detected by chemiluminescence.

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Western Blot - Anti-DDB1 Antibody (A83757)

DDB1 expression in NIH3T3 (A) and NSO (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DDB1 Antibody (A83757) at 0.01µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-DDB1 Antibody (A83757)

DDB1 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-DDB1 Antibody (A83757) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Immunohistochemistry - Anti-DDB1 Antibody (A83757)

Negative control for DDB1 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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