Mouse monoclonal [MR-1] antibody to DC-SIGN (FITC).
Specificity
This antibody recognises human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a ~45 kDa C-type lectin that binds ICAM-3 also known as CD209. CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro.CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's. Mouse anti Human CD209 antibody, clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD209 antibody, clone MR-1 recognizes human DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a ~45 kDa C-type lectin that binds ICAM-3 also known as CD209. CD209 is primarily expressed on a population of immature dendritic cells in peripheral tissues and on immature myeloid monocyte-derived DC's in vitro.CD209 is involved in dendritic cell (DC) migration and the initial interaction between DC's and naive T lymphocytes. CD209 also binds HIV-1 gp120 and plays a key role in the dissemination of HIV-1 by DC's.Mouse anti Human CD209 antibody, clone MR-1 is reported to partially block the functional activity of DC-SIGN (Melero et al.).
Synonyms
C-type lectin domain family 4 member L, CD209, CD209 antigen, CLEC4L, DC-SIGN1, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
