DC-SIGN expression in Human Bone Marrow lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-DC-SIGN Antibody (A83044) at 0.3µg/ml and detected by chemiluminescence.
DC-SIGN expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-DC-SIGN Antibody (A83044) at 6µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for DC-SIGN expression in Human Placenta analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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