Anti-Cytokeratin 5 Antibody (A14122)

Name: Anti-Cytokeratin 5 Antibody
Brand: Antibodies.com
SKU: A14122
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to Cytokeratin 5 for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-Cytokeratin 5 Antibody

Description

Rabbit polyclonal antibody to Cytokeratin 5.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:2,000-1:6,000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 5 (KRT5) (NP_000415.2).

Sequence

MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin, type II cytoskeletal 5, Keratin-5, KRT5, Type-II keratin Kb5

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cytokeratin 5 Antibody (A14122)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 5 Antibody (A14122) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Cytokeratin 5 Antibody (A14122)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Cytokeratin 5 Antibody (A14122)

Immunofluorescence analysis of L929 cells using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-Cytokeratin 5 Antibody (A14122)

Immunoprecipitation analysis of 200µg extracts of A-431 cells using 3µg of Anti-Cytokeratin 5 Antibody (A14122). This Western blot was performed on the immunoprecipitate using Anti-Cytokeratin 5 Antibody (A14122) at a dilution of 1:5000.

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