Anti-Cytokeratin 19 Antibody [ARC0272] (A308569)

Name: Anti-Cytokeratin 19 Antibody [ARC0272]
Brand: Antibodies.com
SKU: A308569
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0272] antibody to Cytokeratin 19 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cytokeratin 19 Antibody [ARC0272]

Description

Rabbit monoclonal [ARC0272] antibody to Cytokeratin 19.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 301-400 of human Cytokeratin 19 (KRT19) (P08727).

Sequence

RTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0272

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CK-19, Cytokeratin-19, K19, Keratin, type I cytoskeletal 19, Keratin-19, KRT19

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (8)

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Western Blot - Anti-Cytokeratin 19 Antibody [ARC0272] (A308569)

Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 19 Antibody [ARC0272] (A308569) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC0272] (A308569)

Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-Cytokeratin 19 Antibody [ARC0272] (A308569) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC0272] (A308569)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cytokeratin 19 Antibody [ARC0272] (A308569) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Cytokeratin 19 Antibody [ARC0272] (A308569)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cytokeratin 19 Antibody [ARC0272] (A308569) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.