Mouse monoclonal [C-04] antibody to Cytokeratin 18.
Specificity
This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
SDS-PAGE analysis of Anti-Cytokeratin 18 Antibody [C-04] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Flow cytometric analysis of PFA fixed HeLa cells using Anti-Cytokeratin 18 Antibody [C-04] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).