This antibody recognises cytochrome P450 1B1, also known as CYPIB1. It is a member of the cytochrome P450 superfamily of enzymes. It catalyses the hydroxylation of 17-ß-oestradiol at the C4 position (Jefcoate et al. 2000, Potter et al. 2002). A number of Cytochrome P450 1B1 mutations are associated with primary congenital glaucoma.
Applications
WB, IF
Dilutions
IF: 1:50 - 1:200, WB: 1:500 - 1:2,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human cytochrome P450 1B1.
Approximately 62 kDa in various cell and tissue lysates under reducing conditions
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.02% Sodium Azide.
Storage
Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rabbit anti cytochrome P450 1B1 antibody recognizes cytochrome P450 1B1, also known as CYPIB1. It is a member of the cytochrome P450 superfamily of enzymes. It catalyses the hydroxylation of 17-ß-oestradiol at the C4 position (Jefcoate et al. 2000, Potter et al. 2002). A number of Cytochrome P450 1B1 mutations are associated with primary congenital glaucoma (Rauf et al. 2016).
