Anti-Cyclin E1 (phospho Thr395) Antibody (A94475)

Name: Anti-Cyclin E1 (phospho Thr395) Antibody
Brand: Antibodies.com
SKU: A94475
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395) for WB, IHC and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-Cyclin E1 (phospho Thr395) Antibody

Description

Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).

Specificity

This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr395.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:5000

Reactivity

Human

Immunogen

Synthetic peptide derived from human Cyclin E1 around the phosphorylation site of Thr395 (amino acids 361-410).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

47kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNE, CCNE1, G1/S-specific cyclin-E1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A94475)

Western blot analysis of lysates from HeLa cells treated with Paclitaxel 1uM 60' using Anti-Cyclin E1 (phospho Thr395) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-Cyclin E1 (phospho Thr395) Antibody (A94475)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Anti-Cyclin E1 (phospho Thr395) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.