Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)

Name: Anti-Cyclin E1 (phospho Thr395) Antibody
Brand: Antibodies.com
SKU: A10865
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395) for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cyclin E1 (phospho Thr395) Antibody

Description

Rabbit polyclonal antibody to Cyclin E1 (phospho Thr395).

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1).

Sequence

LLTPP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CCNE, CCNE1, G1/S-specific cyclin-E1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Immunofluorescence - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)

Immunofluorescence analysis of C6 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Cyclin E1 (phospho Thr395) Antibody (A10865)

Immunofluorescence analysis of U2OS cells using Anti-Cyclin E1 (phospho Thr395) Antibody (A10865) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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