Rabbit polyclonal antibody to Cyclin D3 (Phospho-Thr283)
Specificity
The antibody detects endogenous level of Cyclin D3 only when phosphorylated at Threonine 283.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Threonine 283 (T-S-T(p)-P-T) derived from Human Cyclin D3.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Mouse heart tissue using Cyclin D3 (Phospho-Thr283) Antibody #12004.The lane on the left is treated with the antigen-specific peptide.
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