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Anti-Cyclin D1 (phospho Thr286) Antibody (A94375)

Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.com Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.com Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Cyclin D1 (phospho Thr286) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Cyclin D1 (phospho Thr286) Antibody
Description
Rabbit polyclonal antibody to Cyclin D1 (phospho Thr286).
Specificity
This antibody detects endogenous levels of Cyclin D1 only when phosphorylated at Thr286.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Cyclin D1 around the phosphorylation site of Thr286 (amino acids 246-295).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
33kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1, CCND1, G1/S-specific cyclin-D1, PRAD1, PRAD1 oncogene
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A94375)

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30' using Anti-Cyclin D1 (phospho Thr286) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A94375)

Western blot analysis of various cells using Anti-Cyclin D1 (phospho Thr286) Antibody.
Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A0417) - Antibodies.comEnlarge Image

Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A94375)

Western blot analysis of rat musle cells using Anti-Cyclin D1 (phospho Thr286) Antibody.
Publishing research using Anti-Cyclin D1 (phospho Thr286) Antibody (A94375)? Please let us know so that we can list the citation on this page.

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