Western Blot - Anti-Cyclin D1 (phospho Thr286) Antibody (A305286)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin D1 (phospho Thr286) Antibody (A305286) at 1:1,000 dilution or Cyclin D1 antibody (A11310). NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded rat eye using Anti-Cyclin D1 (phospho Thr286) Antibody (A305286) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cyclin D1 (phospho Thr286) Antibody (A305286) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse eye using Anti-Cyclin D1 (phospho Thr286) Antibody (A305286) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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