This antibody recognises cyclin D1, a 295 amino acid, ~36 kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle.Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis.
Applications
WB, IHC-P, IP, Flow Cytometry, ELISA, IHC-Fr
Dilutions
FC 1: Neat, IHC-P 2: 1:1,000 - 1:10,000, WB: 1:500, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human, Rat
Immunogen
Human Cyclin D1.
Host
Mouse
Clonality
Monoclonal
Clone ID
CD1.1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Cyclin D1 antibody, clone CD1.1 recognizes cyclin D1, a 295 amino acid, ~36 kDa nuclear protein which belongs to the highly conserved cyclin family. D type cyclins are predominantly expressed in the G1 phase of the cell cycle.Cyclin D1 study is of interest in various forms of malignancy and over expression has been linked to poor prognosis (Bartkova et al. 1995).