Anti-Cyclin D1 Antibody [CCND1/3548] - BSA and Azide free
Description
Mouse monoclonal [CCND1/3548] antibody to Cyclin D1.
Specificity
This antibody recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Applications
ELISA
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 115-270 protein, of human CCND1 protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
CCND1/3548
Isotype
IgG2b
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Cyclin D1 Antibody [CCND1/3548] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Anti-Cyclin D1 Antibody [CCND1/3548] - BSA and Azide free (A253040)
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Cyclin D1 Antibody [CCND1/3548]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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