Anti-Cyclin D1 Antibody (A13423)

Name: Anti-Cyclin D1 Antibody
Brand: Antibodies.com
SKU: A13423
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Cyclin D1 for WB, ICC/IF, IP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cyclin D1 Antibody

Description

Rabbit polyclonal antibody to Cyclin D1.

Applications

WB, ICC/IF, IP, ELISA

Positive Control

HeLa, SH-SY5Y, U-87MG, SW480, HT-1080, Mouse kidney, Rat kidney.

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration)

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to amino acids 200-295 of human Cyclin D1.

Sequence

PSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Predicted MW

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, BCL1, CCND1, G1/S-specific cyclin-D1, PRAD1, PRAD1 oncogene

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Cyclin D1 Antibody (A13423)

Cyclin D1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Cyclin D1 Antibody (A13423) at a dilution of 1:1000 and detected with chemiluminescence.

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Western Blot - Anti-Cyclin D1 Antibody (A13423)

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Immunoprecipitation - Anti-Cyclin D1 Antibody (A13423)

Cyclin D1 expression in 200 µg extracts of HepG2 cells analyzed by western blot following immunoprecipitation, both using Anti-Cyclin D1 Antibody (A13423) at 3 µg and a dilution of 1:1000, respectively.

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