The antibody detects endogenous level of total Cyclin B1 protein.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around aa.145~149 (A-F-S-D-V) derived from Human Cyclin B1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Predicted MW
60kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin B1 (Ab-147) Antibody #21540 (left) or the same antibody preincubated with blocking peptide (right).
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] (A250460) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250460_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [CCNB1/1098] - BSA and Azide free (A253640) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253640_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] (A250459) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250459_1.jpg?profile=product_alternative)
![SDS-PAGE - Anti-Cyclin B1 Antibody [V92.1] - BSA and Azide free (A253639) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253639_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Cyclin B1 Antibody [SPM619] - BSA and Azide free (A253641) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253641_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-Cyclin B1 Antibody [V152] (A285847) - Antibodies.com](https://cdn.antibodies.com/image/catalog/285/A285848_1.jpg?profile=product_alternative)