This antibody recognizes CD184, a 45 kDa G-protein-linked CXC chemokine receptor widely expressed on blood and tissue cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
CP-MAC infected Sup-T1 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
12G5
Isotype
IgG2a
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD184 Antibody [12G5] (APC) (10µl reagent per 100µl of peripheral whole blood).
Separation of human CD184 positive lymphocytes (red-filled) from monocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD184 Antibody [12G5] (APC) (10µl reagent per 100µl of peripheral whole blood).
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