Anti-CX3CR1 Antibody (A14239)

Western blot analysis of extracts of various cell lines, using Anti-CX3CR1 Antibody (A14239) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CX3CR1 Antibody (A14239) at a dilution of 1:20 (40x lens). Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CX3CR1 Antibody (A14239) at a dilution of 1:20 (40x lens). Perform microwave antigen retrieval with 50 mM Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol.