This antibody recognises rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and by regulatory T-lymphocytes.
Applications
WB, Flow Cytometry, ELISA
Dilutions
FC 1: 1:10 - 1:20, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Rat
Immunogen
Purified rCTLA-4hIg fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
WKH203
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD152 antibody, clone WKH203 recognizes rat CD152, also known as cytoxic-T-lymphocyte antigen-4 (CTLA-4), which is similar in structure to CD28 and also binds ligands CD80 and CD86. CD152 is expressed by activated T-lymphocytes and by regulatory T-lymphocytes (Lin and Hunig 2003).
Synonyms
CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4