Recombinant rabbit monoclonal [DM51] antibody to CTLA4.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM51
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
Anti-CTLA4 Antibody [DM51] - BSA and Azide free (A318645)
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human CTLA4 Protein (Fc Chimera 6xHis Tag) (A318391) can bind Anti-CTLA4 Antibody [DM51] - Azide free (A318645) in a linear range of 1-100 µg/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human CTLA-4 (B) were surface stained with Anti-CTLA4 Antibody [DM51] - Azide free (A318645) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-CTLA4 Antibody [DM51] - Azide free (A318645) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different rabbit monoclonal Anti-CTLA-4 Antibodies by titration of different concentrations onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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