Mouse monoclonal [BNI3] antibody to CTLA4 (PE-Cyanine 5).
Specificity
This antibody recognizes an extracellular domain of human CD152, an approximately 45 kDa type I transmembrane protein serving as a negative regulator of T cell responses.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human CD152-IgG heavy chain fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
BNI3
Isotype
IgG2a
Conjugate
PE-Cyanine 5
Excitation: 496nm, Emission: 667nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow cytometric analysis of human PHA stimulated peripheral blood mononulcear cells stained with Anti-CD152 Antibody [BNI3] (PE-Cyanine 5) (4µl reagent per milion cells in 100µl of cell suspension).
Separation of human PHA stimulated peripheral blood mononulcear cells stained with Anti-CD152 Antibody [BNI3] (PE-Cyanine 5) (4µl reagent per milion cells in 100µl of cell suspension, red-filled) from PHA stimulated peripheral blood mononulcear cells stained with Mouse IgG2b [MPC-11] (PE-Cyanine 5) (Isotype Control) (concentration in sample is 5µg/ml, same as Anti-CD152 Antibody [BNI3] (PE-Cyanine 5) concentration, black-dashed) in flow cytometry analysis of peripheral blood mononulcear cell suspension.
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