Western Blot - Anti-CRMP2 (phospho Thr514) Antibody (A90916)
Western blot analysis of extracts of various cell lines, using Anti-CRMP2 (phospho Thr514) Antibody (A90916) at 1:1,000 dilution or DPYSL2 antibody (A14570). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.
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