Rabbit polyclonal antibody to CREB (Phospho-Thr100)
Specificity
The antibody detects endogenous levels of CREB only when phosphorylated at threonine 100.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 100 (S-G-T(p)-Q-I) derived from Human CREB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
cAMP-responsive element-binding protein 1, CREB-1, CREB1, Cyclic AMP-responsive element-binding protein 1
Western blot analysis of extracts from HUVEC cells, treated with etoposide (25uM, 24hours), using CREB (Phospho-Thr100) antibody #12133. The lane on the right is treated with the synthesized peptide.
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