Anti-CREB (phospho Ser133) Antibody (A10929)

Name: Anti-CREB (phospho Ser133) Antibody
Brand: Antibodies.com
SKU: A10929
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to CREB (phospho Ser133) for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CREB (phospho Ser133) Antibody

Description

Rabbit polyclonal antibody to CREB (phospho Ser133).

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).

Sequence

RPSYR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

cAMP-responsive element-binding protein 1, CREB-1, CREB1, Cyclic AMP-responsive element-binding protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CREB (phospho Ser133) Antibody (A10929)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-CREB (phospho Ser133) Antibody (A10929) at 1:1,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10929)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CREB (phospho Ser133) Antibody (A10929) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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