Anti-COX2 Antibody (A13409)

Name: Anti-COX2 Antibody
Brand: Antibodies.com
SKU: A13409
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to COX2 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-COX2 Antibody

Description

Rabbit polyclonal antibody to COX2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-111 of COX2/PTGS2 (NP_000954.1).

Sequence

ANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLID

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

69 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

COX-2, COX20, Cyclooxygenase-2, Cytochrome c oxidase assembly protein COX20, mitochondrial, FAM36A, PGH synthase 2, PGHS-2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, PTGS2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-COX2 Antibody (A13409)

Western blot analysis of various lysates, using Anti-COX2 Antibody (A13409) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Western Blot - Anti-COX2 Antibody (A13409)

Western blot analysis of Mouse lung, using Anti-COX2 Antibody (A13409) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-COX2 Antibody (A13409)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-COX2 Antibody (A13409) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-COX2 Antibody (A13409)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-COX2 Antibody (A13409) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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