Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
COX IV-1, COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase subunit IV isoform 1
Western blot analysis of extracts of various cell lines, using Anti-COX IV Antibody (A9916) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence analysis of HepG2 cells using Anti-COX IV Antibody (A9916) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-COX IV Antibody (A9916). This Western blot was performed on the immunoprecipitate using Anti-COX IV Antibody (A9916) at a dilution of 1:1000.
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