Mouse monoclonal (6B1) antibody to Complement C3 alpha chain.
Applications
WB, ELISA
Dilutions
WB: 1:5,000-1:10,000
Reactivity
Human
Immunogen
Recombinant fragment corresponding to amino acids 668-741 of human C3 N-terminal anaphylatoxin construct (NP_000055.2), expressed in and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
6B1
Isotype
IgM
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
185 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1
Western Blot - Anti-Complement C3 alpha chain Antibody [6B1] (A85334)
Western blot analysis of Anti-a Subunit Of Complement Component 3 Antibody on human serum samples. Blot of 0.1 µg purified human C3 protein (Lane 1), 10 µg normal human serum proteins (Lane 2) was probed with Anti-a Subunit Of Complement Component 3 Antibody (1:5,000). The Anti-a Subunit Of Complement Component 3 Antibody binds strongly and cleanly to a band at about 115 kDa which represents the intact a subunit of C3 and various proteolytic bands at approximately 68 and 48 kDa. Bands at 190 kDa and above are likely the pro-C3 and its glycosylated form.
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