Anti-Collagen I Antibody (A329273)

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$355

Rabbit polyclonal antibody to Collagen I for WB, IHC-P and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Collagen I Antibody

Description

Rabbit polyclonal antibody to Collagen I.

Applications

WB, IHC-P, ICC/IF

Positive Control

U-138 MG.

Dilutions

WB: 1:1,000-1:5,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A recombinant fusion protein corresponding to amino acids 26-161 of human Collagen I.

Sequence

GQVEGQDEDIPPITCVQNGLRYHDRDVWKPEPCRICVCDNGKVLCDDVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

220 kDa

Predicted MW

138 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha-1 type I collagen, COL1A1, Collagen alpha-1(I) chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Collagen I Antibody (A329273)

Collagen I expression in U-138 MG cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Collagen I Antibody (A329273) at a dilution of 1:2000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-Collagen I Antibody (A329273)

Collagen I expression in human colon tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Collagen I Antibody (A329273) at a dilution of 1:200.

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Immunofluorescence - Anti-Collagen I Antibody (A329273)

Collagen I expression in Human colon tissue analyzed by immunofluorescence. Staining was performed with Anti-Collagen I Antibody (A329273) at a dilution of 1:200 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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