Anti-Collagen I Antibody (A329272)

Name: Anti-Collagen I Antibody
Brand: Antibodies.com
SKU: A329272
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Collagen I for WB, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Collagen I Antibody

Description

Rabbit polyclonal antibody to Collagen I.

Applications

WB, ICC/IF, ELISA

Positive Control

SK-N-SH.

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

A recombinant fusion protein corresponding to amino acids 1359-1464 of human Collagen I.

Sequence

STEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTVDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPVCFL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

220 kDa

Predicted MW

139 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Alpha-1 type I collagen, COL1A1, Collagen alpha-1(I) chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Collagen I Antibody (A329272)

Collagen I expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Collagen I Antibody (A329272) at a dilution of 1:3000 and detected with chemiluminescence. Negative control: HT-29 cells

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Immunofluorescence - Anti-Collagen I Antibody (A329272)

Collagen I expression in NIH/3T3 cells analyzed by immunofluorescence. Staining was performed with Anti-Collagen I Antibody (A329272) at a dilution of 1:250 followed by Cy3 Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).

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