Rabbit polyclonal antibody to cofilin (Phospho-Ser3)
Specificity
The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from COLO205 cells using cofilin (Phospho-Ser3) Antibody #11139 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3) Antibody #11139 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-Cofilin Antibody [ARC2615] (A307020) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307020_1.jpg?profile=product_alternative)
