Cofilin 2 expression in Human Skeletal Muscle lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Cofilin 2 Antibody (A83312) at 0.003µg/ml and detected by chemiluminescence.
Cofilin 2 expression in Rat Skeletal Muscle lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Cofilin 2 Antibody (A83312) at 0.001µg/ml and detected by chemiluminescence.
Cofilin 2 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress Cofilin 2 and incubated with Anti-Cofilin 2 Antibody (A83312). Control: Mock-transfected HEK293 cell lysate incubated with Anti-Cofilin 2 Antibody (A83312) (first lane).
Cofilin 2 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Cofilin 2 Antibody (A83312) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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