Anti-CNPase Antibody (A85412)

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Anti-CNPase Antibody (A85412), dilution 1:1,000, in green, and co-stained with Anti-GFAP Antibody [5C10] (A85422), dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The CNP antibody stains the myelin producing cells, oligodendrocytes in this part of the CNS, while the GFAP antibody stains astroglia.

Western blot analysis of different tissue lysates using Anti-CNPase Antibody (A85412), dilution 1:5,000, in red. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, [Lane 5] mouse spinal cord, and [Lane 6] cow spinal cord. Double bands at 46 and 48 kDa mark correspond to the two isotypes of the CNP protein. The blot was simultaneously probed with Anti-Calretinin Antibody (A85366), dilution 1:2,000, in green. The single band at 29 kDa corresponds to the calretinin protein.

Immunofluorescent analysis of Anti-CNPase Antibody (A85412) and an appropriate secondary antibody on a 1mm section of rat brain.

Blot of rat brain tissue homogenates blotted with Anti-CNPase Antibody, at a dilution of 1:5,000) using LiCor infrared imager. Anti-CNPase Antibody binds strongly and specifically to a band at ~48 kDa. Numbers at left show positions of protein standards of indicated molecular weight in kDa.