Clusterin expression in Mouse (A) and Rat (B) Brain lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Clusterin Antibody (A84594) at 0.1µg/ml (A) or 0.3µg/ml (B) and detected by chemiluminescence.
Clusterin expression in PC12 suspension cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Clusterin Antibody (A84594) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
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