Anti-Clusterin Antibody [3R3/2] (A279314)

Anti-Clusterin Antibody [3R3/2]

Mouse monoclonal [3R3/2] antibody to Clusterin.

This antibody recognises clusterin, an ubiquitously expressed protein of two 40 kDa chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localised to the nucleus and a pro-survival secreted form.Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas.Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer's disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells.

Flow Cytometry, ELISA, IHC-Fr, WB

Human

Glomerular basement membrane from a post-mortem kidney.

Mouse

Monoclonal

3R3/2

IgG1

Protein A affinity chromatography of ascites.

Liquid

Supplied in Borate Buffered Saline with 0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human clusterin antibody, clone 3R3/2 recognizes clusterin, an ubiquitously expressed protein of two 40 kDa chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localised to the nucleus and a pro-survival secreted form. Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas.Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer's disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells.

Aging-associated gene 4 protein, Apo-J, APOJ, Apolipoprotein J, CLI, CLU, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, KUB1